FastQCFastQC Report
Mon 12 Jun 2023
M.Las.020.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.020.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40009945
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3141570.7851972803261789No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA558420.139570299334328No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC498790.12466650479024653No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG496820.124174127207623No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA495530.12385170736925531No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG484990.12121736233328989No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT477020.11922535759546783No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAG470950.11770823479012531No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAA460990.11521885371249573No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA455640.11388168616577703No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG439590.10987018352562093No Hit
TCTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTC437010.10922534384888558No Hit
GTGAAGATACGTTGTTAGGTGCTCCATTTTTTTTCCCATTGAGGCCGAAC435630.10888042960318992No Hit
GTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGA405910.10145227642777314No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGC401570.10036754611884621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)