FastQCFastQC Report
Mon 12 Jun 2023
M.Las.020.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.020.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37575961
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14474853.8521569681211876No Hit
GTGAAGATACGTTGTTAGGTGCTCCATTTTTTTTCCCATTGAGGCCGAAC696750.18542439939194102No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA561860.14952644857173447No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG549430.1462184826091341No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT526690.14016674117795683No Hit
GGGCGATCTCGTAGTTCTTGGTCTGTGAAGATACGTTGTTAGGTGCTCCA518350.13794723706467546No Hit
GCTGGTTTTAAGAATGAGTAATTGCCCTTCTCCGACCCTTACTGCCCAAC514870.13702111304618397No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC498120.13256347588821482No Hit
GTCCTATTGAGAGAGACCATCCAAGTATGCACGGGCCAACTGGGAACCGA435350.11585864696847008No Hit
CTCGTAGTTCTTGGTCTGTGAAGATACGTTGTTAGGTGCTCCATTTTTTT418660.11141697746599216No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA403390.1073532091434734No Hit
CCTGAATCGCCCCATCTATCCTCCTGAGGAGGAGTTTGTTTTCAAACCCC393160.10463072388221821No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA391620.10422088739127656No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAA387910.10323355402673533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)