FastQCFastQC Report
Mon 12 Jun 2023
M.Las.030.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.030.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31322281
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT648060.20690064047378925No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA621020.198267808145901No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG579660.18506315041359855No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAG560150.1788343575616348No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA547490.17479250633119597No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAA515450.16456336625036982No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG508460.1623317280117626No Hit
GTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGA490570.15662013887175075No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA484910.15481311849542503No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGC483950.15450662740686094No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC478720.15283688949728788No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA437760.13975993638522047No Hit
TCTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTC437230.13959072776340906No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG430090.1373112002922137No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG406760.12986282831700538No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC379190.12106078736730572No Hit
CGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTAGCA371000.11844603526799341No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAG357260.11405938156292No Hit
GGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAA351430.11219808672299442No Hit
GCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGA350470.11189159563443032No Hit
GAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTGC340630.10875006197664851No Hit
CCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGG331510.10583839663528974No Hit
GCCAAAATAACCGTGAGCAGCTACGATATTATAAGTTTCTTCCTCTTGAC325400.10388770856119961No Hit
CGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATC322800.1030576285296719No Hit
GCAGCTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGC319650.102051954645321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)