FastQCFastQC Report
Mon 12 Jun 2023
M.Las.030.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.030.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31322281
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA817860.26111125176356087No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA590970.18867399855074413No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTCATG548540.17512773095931297No Hit
CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG537570.17162543174936717No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG511490.163299090510043No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC479660.1531369953548402No Hit
GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTG449540.1435208374511422No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCAT436860.13947260098969166No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT421870.13468687034638377No Hit
GGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAA386890.12351910130683011No Hit
GGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATAT386430.12337224099355983No Hit
CAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGT362130.11561418531428155No Hit
ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA356270.11374331262783831No Hit
GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACAGAAAATG343660.10971742447492888No Hit
CGGCGGCTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT331450.1058192409422545No Hit
GTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGA329630.10523818492018511No Hit
GGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTT326800.10433467473202224No Hit
GGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAAG324890.10372488517039993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)