FastQCFastQC Report
Mon 12 Jun 2023
M.Las.030.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.030.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42453422
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA908310.21395448404606818No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC659330.1553066794003084No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG625860.1474227448614154No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA593130.13971311900369304No Hit
CCAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAG497580.1172060994282157No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA474090.11167297656240761No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC471160.11098280840588068No Hit
CCCTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAG441420.10397748384099637No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT439070.10342393600214371No Hit
CCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGG434600.1023710173469644No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA427360.10066561889875449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)