FastQCFastQC Report
Mon 12 Jun 2023
M.Las.030.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.030.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50124538
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14606532.9140478062860153No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA803250.1602508535839273No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC773050.15422586039596017No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA701100.139871613380257No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG616730.1230395380402309No Hit
TCTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTC609010.12149937421867112No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA586930.11709434608654148No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT576580.11502948914960574No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG568360.11338957378519879No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAG564930.11270527820126741No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGC543030.10833616062456276No Hit
GTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGA535490.10683190735842792No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG529680.10567279443054417No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAA526490.10503637958717944No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC510420.10183036500007242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)