FastQCFastQC Report
Mon 12 Jun 2023
M.Las.030.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.030.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50124538
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1893870.37783290890381876No Hit
CTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1158570.2311382899928175No Hit
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA1042040.2078901954168635No Hit
CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG928070.18515282874028685No Hit
GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTG696800.13901375011177158No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA689200.1374975266604951No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT625760.12484105090405023No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC600380.1197776625891295No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT591930.11809186151501287No Hit
CCTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579950.11570181454839544No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTCATG554500.11062446101747611No Hit
CGGCGGCTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT519860.10371367412902639No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG516660.1030752642548047No Hit
ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA510410.10182836996921547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)