FastQCFastQC Report
Mon 12 Jun 2023
M.Las.060.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.060.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43632443
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA718200.16460228917276074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA684650.15691305664457064No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA584920.1340562113379716No Hit
CCCTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAG544870.12487726162846303No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC499850.11455925124339245No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG491030.11253781962197258No Hit
GTCCTATTGAGAGAGACCATCCAAGTATGCACGGGCCAACTGGGAACCGA448910.10288445228702871No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT438400.10047569419846603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)