FastQCFastQC Report
Mon 12 Jun 2023
M.Las.060.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.060.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43632443
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC654850.150083276336372No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCAT507310.11626898819302875No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT437010.10015712390892254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)