FastQCFastQC Report
Mon 12 Jun 2023
M.Las.060.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.060.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38706276
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26201786.769387992789593No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC955760.24692636408627894No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA780300.20159521417146925No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA739760.19112146050940163No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG663470.17141147859329064No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGC644200.166432957797335No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG580960.15009452213899369No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAG577060.14908693360218894No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCGATTGCAATCTCGTATGCCGT574170.14834028466081314TruSeq Adapter, Index 2 (96% over 32bp)
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG522550.1350039461300798No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAA518520.1339627713087149No Hit
TCTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTC517670.1337431686788985No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC504640.13037678954183038No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT494090.12765113337175604No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAG479110.12378095996628558No Hit
GTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGA463490.1197454386983651No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA450240.1163222212335798No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA435500.11251405327652808No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC420310.1085896251036912No Hit
GCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGA417920.10797215417985445No Hit
GGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAA416740.10766729405846225No Hit
GCCAGGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAA416580.10762595709284974No Hit
GTGAAGATACGTTGTTAGGTGCTCCATTTTTTTTCCCATTGAGGCCGAAC413490.10682763694445832No Hit
CGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTAGCA409830.10588205385607234No Hit
GTGCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTA394890.10202221469200498No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG392180.10132206983694324No Hit
CCAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAG390500.10088803169801197No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)