FastQCFastQC Report
Mon 12 Jun 2023
M.Las.060.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.060.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38706276
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3828290.9890618255292759No Hit
CTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1899070.49063619553583504No Hit
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA1132610.2926166288898472No Hit
CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG1122510.2900072329355581No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT994220.256862737195384No Hit
CCTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT958550.2476471774241469No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT884890.22861667188029144No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA683130.17649075824292682No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680310.17576219422400646No Hit
GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTG639060.16510500777703335No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTGTATCTTCGTGTAGATCTCGGTG608370.15717606106048537Illumina Single End Sequencing Primer (100% over 27bp)
ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA593050.15321804660308835No Hit
TCTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATA562150.14523484511917395No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC542420.14013748054708233No Hit
GGGGGGACCACCACGGCTCCTCTCTTCTCGATAATCCATACATCCCTTAT541820.13998246692603547No Hit
GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACAGAAAATG483760.1249823155293989No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477250.12330041774104024No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC454880.11752099323634234No Hit
CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG451930.11675884293286184No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG451720.11670458816549543No Hit
CTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTT448620.11590368445675323No Hit
CTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGC436720.11282924763932341No Hit
ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGAC432150.11164856055901633No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCAT425590.10995374496890375No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC419900.10848369912930915No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTCATG408560.1055539416915231No Hit
GCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTT391030.10102496039660339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)