FastQCFastQC Report
Mon 12 Jun 2023
M.h.000.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.000.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41442363
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2256990.5446093891895112No Hit
GTGAAGATACGTTGTTAGGTGCTCCATTTTTTTTCCCATTGAGGCCGAAC546860.13195676124935252No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT479660.1157414696647486No Hit
GCTGGTTTTAAGAATGAGTAATTGCCCTTCTCCGACCCTTACTGCCCAAC476860.11506583251539011No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG424420.10241211390383315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)