FastQCFastQC Report
Mon 12 Jun 2023
M.h.000.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.000.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62800278
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAGGAGAGTGAGGGGAAGAGTCAACTACGTCGAACCTCGGCCCGATC2164990.3447421044855884No Hit
GTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAGTCAACTACGTCGAACC1905430.30341107725669625No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA1836870.29249392813197417No Hit
CTTTGATGACTCAAGGTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAGT1782220.28379173735504803No Hit
CCCTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAG1575470.250869908569513No Hit
CCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCG1518190.24174892983753987No Hit
GCTTTGATGACTCAAGGTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAG1497840.23850849832225263No Hit
CGCCTTTTGAAGCGCCAGTCGCGTAGGGCGACCGGGCCAGGCCGAGTCAG1340860.21351179368983048No Hit
GCCTTTTGAAGCGCCAGTCGCGTAGGGCGACCGGGCCAGGCCGAGTCAGA1282450.20421087944865468No Hit
GGAAGAGTCAACTACGTCGAACCTCGGCCCGATCATCCAATTCGCTCCAA1230700.1959704700670274No Hit
GTCGCGTAGGGCGACCGGGCCAGGCCGAGTCAGAAAGGCTTTGATGACTC1219640.19420933136633567No Hit
GGGAAGAGTCAACTACGTCGAACCTCGGCCCGATCATCCAATTCGCTCCA1206080.1920501052559035No Hit
CTCCAACAGACAGGCATGGTTCTGTAGTCAAAGCAACTTCGTCACTTTCG1203270.1916026550073552No Hit
GGGAAAGGAGAGTGAGGGGAAGAGTCAACTACGTCGAACCTCGGCCCGAT1124480.1790565322019753No Hit
GTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGAA1104250.1758352088823556No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTAAA1023650.16300087079232356No Hit
GCCAGGCCGAGTCAGAAAGGCTTTGATGACTCAAGGTTCATATTAGGGAA989980.15763942955793922No Hit
GCCCGATCATCCAATTCGCTCCAACAGACAGGCATGGTTCTGTAGTCAAA950520.151356017882596No Hit
CTGGAAGTTTCCACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG842060.13408539369841643No Hit
CTCAAGGTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAGTCAACTACGT793460.12634657445306213No Hit
CGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCGG788430.12554562258466434No Hit
GGGGAAGAGTCAACTACGTCGAACCTCGGCCCGATCATCCAATTCGCTCC747640.11905042840733922No Hit
CCCGACTCGCAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCGCGACGG742800.11827973118208172No Hit
CTCGGCCCGATCATCCAATTCGCTCCAACAGACAGGCATGGTTCTGTAGT709620.1129963150800065No Hit
CTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAGGG702020.11178612935439554No Hit
GCCGAGTCAGAAAGGCTTTGATGACTCAAGGTTCATATTAGGGAAAGGAG701790.11174950531269942No Hit
GTCAGAAAGGCTTTGATGACTCAAGGTTCATATTAGGGAAAGGAGAGTGA676860.10777977766276768No Hit
CTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAGGGCGA674480.10740079844869475No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA658940.10492628710974815No Hit
CAGTCGCGTAGGGCGACCGGGCCAGGCCGAGTCAGAAAGGCTTTGATGAC645850.10284190143234717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)