FastQCFastQC Report
Mon 12 Jun 2023
M.h.000.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.000.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62800278
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCGCCTGCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCTACGCAG2851370.4540377990046477No Hit
CGATCGCCTGCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCTACGCAGAG2269640.3614060434573236No Hit
ACACGATCGCCTGCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCTACGCA1512420.24083014409585893No Hit
GGAAGGCTTTCTGCGTTCAGTCAATCTACGCAGAGTGAATCGGTCCCTAA1423360.22664867821126522No Hit
AACACGATCGCCTGCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCTACGC997910.1589021628216359No Hit
GGCTTTCTGCGTTCAGTCAATCTACGCAGAGTGAATCGGTCCCTAAGGAA988210.157357583671843No Hit
AGTCAATCTACGCAGAGTGAATCGGTCCCTAAGGAACCCCCGAAAGGGCT932840.14854074372091156No Hit
GTCAATCTACGCAGAGTGAATCGGTCCCTAAGGAACCCCCGAAAGGGCTG915410.14576527830020117No Hit
AAACACGATCGCCTGCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCTACG850200.13538156630453133No Hit
AGGCTTTCTGCGTTCAGTCAATCTACGCAGAGTGAATCGGTCCCTAAGGA831900.13246756646523125No Hit
GCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCTACGCAGAGTGAATCGGT818280.13029878625696528No Hit
CGAAAGTGACGAAGTTGCTTTGACTACAGAACCATGCCTGTCTGTTGGAG808040.12866822022666843No Hit
GCTTTCTGCGTTCAGTCAATCTACGCAGAGTGAATCGGTCCCTAAGGAAC777120.12374467514299856No Hit
GTGGAAGGCTTTCTGCGTTCAGTCAATCTACGCAGAGTGAATCGGTCCCT757320.12059182285785422No Hit
CGCAGAGTGAATCGGTCCCTAAGGAACCCCCGAAAGGGCTGCCGTCCGAT745740.11874788197593648No Hit
GTTGCTTTGACTACAGAACCATGCCTGTCTGTTGGAGCGAATTGGATGAT732050.11656795532019779No Hit
AAAACACGATCGCCTGCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCTAC714230.11373038826356788No Hit
AGTGAATCGGTCCCTAAGGAACCCCCGAAAGGGCTGCCGTCCGATGGGTA689700.10982435459919461No Hit
GAAAAACACGATCGCCTGCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCT676490.10772086072612609No Hit
GAAGGCTTTCTGCGTTCAGTCAATCTACGCAGAGTGAATCGGTCCCTAAG643600.10248362276358076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)