FastQCFastQC Report
Mon 12 Jun 2023
M.h.010.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.010.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38568244
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2874190.7452218980983422No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT557030.14442710951527896No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG472360.12247381550479716No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA423340.10976387724574653No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA404810.10495940650033224No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAG387540.10048162939437948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)