FastQCFastQC Report
Mon 12 Jun 2023
M.h.010.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.010.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38568244
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA499890.12961181224636517No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420410.10900418489366538No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG408250.10585133199219544No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA407780.10572947007906298No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCAT390500.10124910016644781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)