FastQCFastQC Report
Mon 12 Jun 2023
M.h.010.5.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.010.5.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45954496
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAGTTTACCTACTACTGTACCGGCGTGAATATGATCTCCACCAGACA767690.1670543835362703No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA737500.16048484135262847No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC661930.14404031326989203No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG636030.13840430324815226No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC554930.12075641086347678No Hit
CCCAGGTATTAATTACACGACCTTGACTATCAACTACGGATTGGTTGAAA481230.10471880705644122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)