FastQCFastQC Report
Mon 12 Jun 2023
M.h.010.6.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.010.6.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45875737
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAGTTTACCTACTACTGTACCGGCGTGAATATGATCTCCACCAGACA997660.21747007573960064No Hit
GTTAAGTAGTCATGCATTACGATAGGAACTCCCAACTCTCTGGCAAAGAC902740.1967793999690948No Hit
CTGTTAAGTAGTCATGCATTACGATAGGAACTCCCAACTCTCTGGCAAAG726100.15827538639869698No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG709480.15465255631751487No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC597450.1302322401926753No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTAAGATTCAAGGCGGC527300.11494093272005636No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA476590.10388715934961437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)