FastQCFastQC Report
Mon 12 Jun 2023
M.h.020.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.020.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54792384
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA1060530.1935542720681765No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC828590.1512235715095003No Hit
CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG790390.14425179966617258No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTCATG748580.13662117713293878No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCAT733730.1339109464556242No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA718600.13114961378574072No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG707650.12915116086206435No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT706820.12899967995552084No Hit
GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTG630790.11512366390190286No Hit
CAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGT596390.10884541910058157No Hit
GGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTT589420.10757334449984875No Hit
GGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAA585080.10678126361503089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)