FastQCFastQC Report
Mon 12 Jun 2023
M.h.030.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.030.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40585119
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA739680.18225399314463017No Hit
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA661040.16287743298227117No Hit
CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG524600.12925919965887006No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA463900.11430297888248155No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC453100.11164190500463976No Hit
GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTG423770.1044151182604639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)