FastQCFastQC Report
Mon 12 Jun 2023
M.h.030.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.030.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55930348
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8219321.4695635364185469No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT984410.17600641426368382No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA971040.17361594102722194No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG889780.15908715604630244No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC856050.15305644084317158No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAA810960.144994627961192No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAG805090.14394510829791368No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA804160.14377883005483894No Hit
GTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGA796660.14243787648165537No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA794190.1419962557715536No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA792750.14173879268550232No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGC671500.12006004325236812No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG625370.11181228480824043No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG616690.11026035453954265No Hit
TCTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTC610630.10917686405241032No Hit
GCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGA596280.10661117288238578No Hit
CGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTAGCA586710.10490011612300355No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG566560.10129742085638373No Hit
GGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAA562030.10048748489818086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)