FastQCFastQC Report
Mon 12 Jun 2023
M.h.060.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.060.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41183439
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4244731.0306885736278604No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA1168010.2836115750314101No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC1163880.282608744743245No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA1081080.2625035757698623No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG1023840.24860478504478462No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGC944400.22931547800075658No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAG845850.20538595623352385No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT819860.1990751670835454No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG816680.19830301204326334No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC803630.1951342625855019No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG799000.1940100242721352No Hit
GTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGA779860.1893625250674185No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC688110.16708415244292735No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAA672800.16336663871125479No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA663270.16105260175091254No Hit
CGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTAGCA662150.160780647774461No Hit
TCTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTC658600.15991865079552972No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA624640.15167261772383797No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG609510.14799881088123798No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA606280.14721451503843572No Hit
GGCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAG600890.1459057365267626No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAG582350.1414039269522878No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG580650.14099113966660237No Hit
GAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTGC577650.14026269151539286No Hit
CAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGCGGT573730.13931085259781245No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA543900.1320676498142858No Hit
CGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATC535860.13011540876904426No Hit
GCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCGTTCGCTTTCGCGTC524240.1272938862633594No Hit
CCAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAG520780.12645374272896442No Hit
GTGCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCGTTCGCTTTCGCG518590.12592197557858148No Hit
GCCAGGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAA517320.12561359919456944No Hit
CAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGG499610.12131332694192926No Hit
GCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGA481720.11696934780021648No Hit
GAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACCGTG480820.11675081335485364No Hit
GTGCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTA478780.11625546861203118No Hit
CTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACA476730.11575769570870466No Hit
CTAGCATATTGGAAGATCAATCGGCCAAAATAACCGTGAGCAGCTACGAT472460.1147208711734831No Hit
GGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAA471180.11441006662896705No Hit
CAGTTACAGAAGCGACCCCATAGGCGTTCGCTTTCGCGTCTCTCTAAAAT457340.11104949249138713No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA457060.11098150399727424No Hit
GCCAAAATAACCGTGAGCAGCTACGATATTATAAGTTTCTTCCTCTTGAC453250.11005637484523816No Hit
GTTCACGAATACCATCAATATCTACTGGAGGGGCAGCAATGAAGGCGATA450930.10949304160830278No Hit
GTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGCG430470.10452502521705387No Hit
GTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACCGTGAGCAG429060.10418265458598541No Hit
CACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAAT419550.10187347394665122No Hit
GGGGCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGT414930.10075166379378857No Hit
CCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGG412830.10024175008794191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)