FastQCFastQC Report
Mon 12 Jun 2023
M.h.060.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.060.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41183439
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA1507620.36607433390883165No Hit
CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG1132780.2750571655757063No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG1043450.25336640779319086No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA1022140.24819199775909925No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTCATG842920.20467450520584257No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA743440.18051916451173494No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC728160.17680893526157443No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC719510.174708576425587No Hit
GGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAA690290.1676134914328063No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT679070.1648890953472827No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCAT673650.16357303235409748No Hit
ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA672770.16335935422974268No Hit
TCTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATA663500.16110844944250527No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC618770.15024728750797134No Hit
GTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGA603320.14649577952924234No Hit
GGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAAG582740.14149862521194503No Hit
CTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTT569660.13832259127267152No Hit
GCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTT558500.1356127641501721No Hit
CTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC555130.13479447406031342No Hit
GGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGCCTTTACATTGG526600.1278669321423109No Hit
GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTG525830.12767996378350047No Hit
GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACAGAAAATG525570.1276168316103956No Hit
GGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTT503940.12236472044017499No Hit
GGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATAT494510.12007496508487307No Hit
ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGAC492250.1195262008109619No Hit
CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG486270.11807416082955094No Hit
GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACAGAAAATGAATCT477500.1159446640675151No Hit
CAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTATTCG469830.11408226496092276No Hit
CAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGT465240.1129677392895722No Hit
ATTTGGATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTACCA454540.11036960755025824No Hit
CTCCAGTAGATATTGATGGTATTCGTGAACCTGTTTCTGGATCTCTACTT442580.1074655275874363No Hit
GTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGCCTTTACATTGGC422840.10267233875247767No Hit
TGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATA419330.10182005441556252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)