FastQCFastQC Report
Mon 12 Jun 2023
M.h.060.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.060.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32098054
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7134362.222676801528217No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG921060.2869519753440504No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGC887710.27656193736853957No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA857400.26711899730743804No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA808470.251875082520579No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT806650.25130806995339966No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAG779400.24281845871403918No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC769960.23987747045350477No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAA716400.2231911006193709No Hit
GTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGA691990.2155862782211034No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG654450.20389086515961372No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA642730.20023955346327227No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG636970.19844505215175973No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA624100.19443546328384892No Hit
GCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGA604350.1882824422938537No Hit
CGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTAGCA602820.1878057778829832No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG577350.17987071739613872No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAG562070.1751103041947652No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG560960.17476448883785914No Hit
TCTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTC546910.17038727643738152No Hit
GCCAGGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAA532430.16587609952927365No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC518580.16156119620211243No Hit
GAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACCGTG507880.15822766077968467No Hit
GGCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAG499270.1555452551734133No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA478810.14917103697314485No Hit
GTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACCGTGAGCAG473790.14760707923290303No Hit
GCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCGTTCGCTTTCGCGTC473640.1475603474279157No Hit
GGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAA470240.1465010931815368No Hit
GAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTGC462720.14415827202483988No Hit
CGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATC459340.1431052486857926No Hit
GCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAGTA458540.14285601239252696No Hit
GCAGCTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGC450560.14036988036720233No Hit
GCCAAAATAACCGTGAGCAGCTACGATATTATAAGTTTCTTCCTCTTGAC443230.138086252830156No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC437140.13618894154767142No Hit
CAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGG434950.13550665719485674No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA434520.13537269268722646No Hit
CCAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAG419440.13067458855916936No Hit
GTGCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTA418840.13048766133922013No Hit
GCATATTGGAAGATCAATCGGCCAAAATAACCGTGAGCAGCTACGATATT412840.12861838913972792No Hit
GGAAGATCAATCGGCCAAAATAACCGTGAGCAGCTACGATATTATAAGTT408240.12718528045345054No Hit
CAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGCGGT404510.12602321623609955No Hit
GTGCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCGTTCGCTTTCGCG392250.12220367004180377No Hit
CTAGCATATTGGAAGATCAATCGGCCAAAATAACCGTGAGCAGCTACGAT384600.1198203479874512No Hit
GTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTC378710.11798534577828301No Hit
CTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACA377480.11760214497738711No Hit
CCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGG372500.11605064905180855No Hit
GGGGCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGT347700.10832432396057406No Hit
GTTCACGAATACCATCAATATCTACTGGAGGGGCAGCAATGAAGGCGATA345470.1076295777930961No Hit
CATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAGTTGAAAGTAC341490.1063896272340996No Hit
GGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAG334230.10412780787271403No Hit
GGCCAAAATAACCGTGAGCAGCTACGATATTATAAGTTTCTTCCTCTTGA331770.10336140627092222No Hit
AGCAGCTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTG327030.10188468123332335No Hit
GTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGCG322850.10058242160101045No Hit
GTTACAGAAGCGACCCCATAGGCGTTCGCTTTCGCGTCTCTCTAAAATTG321840.1002677607807626No Hit
GCCGAATACACCAGCTACGCCTAACATGTGGAATGGGTGCATAAGGATGT321130.10004656357048936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)