FastQCFastQC Report
Mon 12 Jun 2023
M.h.060.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.060.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32098054
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA1522120.47420943338184923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1284500.4001800233746257No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA1149970.35826782520834444No Hit
CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG893940.2785028650023456No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG834190.25988802934906896No Hit
ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA757950.23613581060085448No Hit
GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTG734630.2288705726521614No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTCATG702370.2188201191262249No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC690940.2152591555861922No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCAT623500.19424853606389972No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT599330.18671848455361187No Hit
GTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGA594670.1852666831453396No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC593440.18488348234444368No Hit
GCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTT578440.18021030184571316No Hit
GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACAGAAAATG570550.17775220890338086No Hit
GGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAA560640.1746647943205529No Hit
ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGAC554070.17261794126210891No Hit
GGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGCCTTTACATTGG525320.1636610119728754No Hit
TCTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATA507210.1580189253840747No Hit
GGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAAG496850.1547913153862848No Hit
GGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATAT495200.15427726553142443No Hit
GGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAG490020.1526634605325295No Hit
CTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTT487570.15190017438440348No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC487280.1518098262280947No Hit
ATTTGGATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTACCA462020.14394019026823246No Hit
CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG457930.14266596971891193No Hit
GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACAGAAAATGAATCT455570.14193072265377832No Hit
GGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTT448760.13980909870735467No Hit
GGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGCCTTTACATT438220.13652541054357997No Hit
CTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432970.1348897973690243No Hit
GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACA422870.13174318916654573No Hit
AATTTGGATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTACC407860.12706689321414938No Hit
GTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGCCTTTACATTGGC399010.12430971671989835No Hit
CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCA394410.12287660803362098No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391050.12182981560190534No Hit
GGAAGCGGCATCCGTTGATGAATGGTTATACAATGGCGGTCCTTATGAGC381320.11879847918506213No Hit
CGGCGGCTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT379090.11810373301758417No Hit
GGATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTACCATGAC367160.11438699679426048No Hit
CAATTCTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTA364960.11370159698778001No Hit
GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCAGCG361280.11255511003875811No Hit
CAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGT360320.11225602648683936No Hit
CATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACAGAAAATGA356440.11104723046450106No Hit
CTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC343910.10714356702122814No Hit
GCGAAAGCGAACGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACT340720.10614973730183144No Hit
GTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC327070.10189714304798664No Hit
AGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTCTG326900.10184418033566771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)