FastQCFastQC Report
Mon 12 Jun 2023
M.h.060.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.060.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40492483
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA1145090.2827907589663No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC1049300.2591345163990067No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA877200.216632800710196No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA667040.16473180960525438No Hit
GCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGG665530.1643589008853816No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG555310.13713903392884055No Hit
CCAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAG553720.13674636845559704No Hit
CGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTAA552260.136385807706581No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA540250.1334198251067982No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAGCCAATGTAAAGGC535590.13226899422295244No Hit
CATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG525940.12988583584760657No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG523060.12917459272625983No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC522140.12894739006249628No Hit
GGCAGCAATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAG497300.12281291814088063No Hit
GCTAGGTCTAGCGGGAAGTTATGAGCATTACGTTCATGCATAACTTCCAT486640.12018033075422913No Hit
GCACGATTAATAATATCCGCCCAGGTATTAATTACACGACCTTGACTATC465600.11498430461772374No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA456110.11264065974911935No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT443810.10960305891836765No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA436150.10771134978311901No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC433390.10702974179182838No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG432920.10691367086577526No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG426740.10538746166788536No Hit
CTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGAATTGTTG415730.1026684384852369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)