FastQCFastQC Report
Mon 12 Jun 2023
M.h.060.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h.060.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40492483
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATAA1191140.29416324012533385No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC926970.22892397090096944No Hit
TCTCGCTTTCTGCAGAATTTGGATGCTTGGGAGTCCCTGATGATTAAATA658140.16253387079275924No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC537340.13270117320293745No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCAT525740.1298364439641797No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTCATG512550.12657904925217847No Hit
CTTAGTTTCCGTCTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC470950.11630553749939217No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT462440.1142039128595794No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG453160.11191212946857322No Hit
GGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAA441170.1089510860571331No Hit
CAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAAGAGGAAGAA436510.10780025517328735No Hit
CATGACTGCAATTTTAGAGAGACGCGAAAGCGAACGCCTATGGGGTCGCT426930.10543438395714089No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT411330.10158181704984601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)