FastQCFastQC Report
Tue 7 Nov 2023
Col.3.1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCol.3.1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19016858
Total Bases950.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCTTAA3789761.9928423507185045Illumina Single End PCR Primer 1 (97% over 35bp)
AATGATACGGCGACCACCGAGATCTACACCTTAAGCCACACTCTTTCCCT606510.318932812139629Illumina RNA PCR Primer (96% over 32bp)
GTACGTGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGT224500.11805315052570724Illumina Single End Sequencing Primer (100% over 33bp)
GTAACCGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCTTAA222650.11708032946346869Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)