This is cutadapt 4.4 with Python 3.9.18 Command line parameters: --minimum-length=20 --max-n 0.1 --quality-cutoff 30,30 --output=/home/jforment/biovice/internal_projects/230904_antserra_chipseq/01-cleandata/BP.3.R1.clean.fq.gz --cores 50 --adapter=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA BP.3.1.fq.gz Processing single-end reads on 50 cores ... Finished in 50.451 s (2.000 µs/read; 30.00 M reads/minute). === Summary === Total reads processed: 25,227,438 Reads with adapters: 660,281 (2.6%) == Read fate breakdown == Reads that were too short: 237,740 (0.9%) Reads with too many N: 0 (0.0%) Reads written (passing filters): 24,989,698 (99.1%) Total basepairs processed: 1,261,371,900 bp Quality-trimmed: 15,632,301 bp (1.2%) Total written (filtered): 1,242,064,420 bp (98.5%) === Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 660281 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-33 bp: 3 Bases preceding removed adapters: A: 36.3% C: 20.2% G: 25.3% T: 18.2% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 3 497624 394178.7 0 497624 4 124672 98544.7 0 124672 5 27963 24636.2 0 27963 6 5946 6159.0 0 5946 7 1699 1539.8 0 1699 8 238 384.9 0 238 9 654 96.2 0 80 574 10 801 24.1 1 16 785 11 417 6.0 1 4 413 12 126 1.5 1 1 125 13 39 0.4 1 0 39 14 9 0.1 1 0 9 15 3 0.0 1 0 3 16 5 0.0 1 1 4 39 1 0.0 3 1 40 18 0.0 3 1 15 2 41 1 0.0 3 0 1 42 1 0.0 3 1 44 5 0.0 3 4 1 46 1 0.0 3 1 47 1 0.0 3 0 1 48 1 0.0 3 0 0 1 50 56 0.0 3 6 41 7 2