This is cutadapt 4.4 with Python 3.9.18 Command line parameters: --minimum-length=20 --max-n 0.1 --quality-cutoff 30,30 --output=/home/jforment/biovice/internal_projects/230904_antserra_chipseq/01-cleandata/Col.2.R1.clean.fq.gz --cores 50 --adapter=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Col.2.1.fq.gz Processing single-end reads on 50 cores ... Finished in 41.141 s (1.936 µs/read; 30.99 M reads/minute). === Summary === Total reads processed: 21,252,268 Reads with adapters: 519,004 (2.4%) == Read fate breakdown == Reads that were too short: 402,965 (1.9%) Reads with too many N: 0 (0.0%) Reads written (passing filters): 20,849,303 (98.1%) Total basepairs processed: 1,062,613,400 bp Quality-trimmed: 25,595,901 bp (2.4%) Total written (filtered): 1,033,039,296 bp (97.2%) === Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Type: regular 3'; Length: 33; Trimmed: 519004 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-33 bp: 3 Bases preceding removed adapters: A: 35.9% C: 20.8% G: 25.1% T: 17.9% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 3 388137 332066.7 0 388137 4 97955 83016.7 0 97955 5 23397 20754.2 0 23397 6 5921 5188.5 0 5921 7 1733 1297.1 0 1733 8 159 324.3 0 159 9 525 81.1 0 52 473 10 671 20.3 1 21 650 11 330 5.1 1 4 326 12 108 1.3 1 3 105 13 28 0.3 1 0 28 14 9 0.1 1 0 9 15 1 0.0 1 0 1 16 1 0.0 1 0 1 40 2 0.0 3 1 1 49 3 0.0 3 0 0 3 50 24 0.0 3 6 12 5 1