FastQCFastQC Report
Tue 7 Nov 2023
Col.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCol.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18507653
Total Bases914.3 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCTTAA3621691.9568607645712828Illumina Single End PCR Primer 1 (97% over 35bp)
AATGATACGGCGACCACCGAGATCTACACCTTAAGCCACACTCTTTCCCT538330.2908688638154173Illumina RNA PCR Primer (96% over 32bp)
GTAACCGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCTTAA213160.11517397694888704Illumina Single End PCR Primer 1 (97% over 35bp)
GTACGTGGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGT208230.11251021401795247Illumina Single End Sequencing Primer (100% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)