FastQCFastQC Report
Wed 9 Apr 2025
Col0.dry.SD.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCol0.dry.SD.2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7870983
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATGCCATCTGGGGG137800.175073431107652TruSeq Adapter, Index 22 (97% over 37bp)
CACACTTTATAATTAAGCATTAAGAGCTCTTGGTAGATTAACATGGGAAG81620.1036973399637631No Hit
CTCTTTCTCAACGGTAGCCTCAACACCAACGTCTCCTTTATTGAGACTAT80550.10233791636953098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)