FastQCFastQC Report
Wed 9 Apr 2025
mA.1.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemA.1.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7601916
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT362580.4769587035689423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG348290.45816081103763845No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT158940.20907886906406226No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT136820.1799809416468164No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG128760.16937835145771146No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA104250.1371364798032496No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC102570.13492651063231953No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT98340.12936212396979918No Hit
AAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCTGC91800.12075902969725001No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT89070.11716782979448864No Hit
CACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAG88770.11677319244253685No Hit
CACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCTGCGCAACTCT83460.10978811131299004No Hit
CAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGAC81720.10749921467166962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)