FastQCFastQC Report
Wed 9 Apr 2025
mA.2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemA.2.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11434445
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG457700.4002817801825974No Hit
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT391310.3422203701185322No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT270670.23671459349360635No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC258900.2264211336886049No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG208200.18208142152942272No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT203730.17817218063491494No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA201400.17613447788677108No Hit
CACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAG196930.1722252369922633No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT185480.16221163335868072No Hit
CAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGAC155940.1363774105345734No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT138840.12142259637437584No Hit
CACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCTGCGCAACTCT130790.11438246456211912No Hit
TCAAAGTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAA127100.11115537308544489No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)