FastQCFastQC Report
Wed 9 Apr 2025
mA.3.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemA.3.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11162785
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG348870.31252953452028326No Hit
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT274150.24559283368800885No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG253360.22696844918181258No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT247030.22129782128742964No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC244020.2186013615777783No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT196330.17587904810493082No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT141370.1266440229745534No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA122600.10982922272533242No Hit
TCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATC117960.10567255393703273No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT116700.10454380336089963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)