FastQCFastQC Report
Wed 9 Apr 2025
mB.1.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemB.1.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724209
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGCGAATCTGGGGG104050.1547393901647019TruSeq Adapter, Index 25 (97% over 37bp)
CACACTTTATAATTAAGCATTAAGAGCTCTTGGTAGATTAACATGGGAAG82730.12303305860957027No Hit
GTCCCACTTGGCCTCTCGCACAACTTCTGTGCTTCCACCACCATCGGTGC77620.11543365174996792No Hit
CCTCATTATTTTTATTTAATATGTATACACGATTGTTCGAAGCTTAACGG73660.10954448322471831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)