FastQCFastQC Report
Wed 9 Apr 2025
mB.1.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemB.1.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724209
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG247500.36807303282809917No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT189040.28113343889221765No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG141070.20979419289317153No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT115200.1713212661890789No Hit
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT112610.16746951202736263No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT101090.15033738540845473No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA93930.13968929282239742No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT90730.13493036876158965No Hit
CACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAG71270.10599016181680254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)