FastQCFastQC Report
Wed 9 Apr 2025
mB.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemB.2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832604
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGTATCATCTAGGGG85880.12569146404504053TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGTATCATCTGGGGG75780.11090939852507185TruSeq Adapter, Index 5 (97% over 38bp)
GTCCCACTTGGCCTCTCGCACAACTTCTGTGCTTCCACCACCATCGGTGC72510.10612352186662655No Hit
CACACTTTATAATTAAGCATTAAGAGCTCTTGGTAGATTAACATGGGAAG72230.1057137220304294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)