FastQCFastQC Report
Wed 9 Apr 2025
mB.2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemB.2.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832604
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325700.47668502374790045No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT154220.22571189549401663No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG146090.2138130645358636No Hit
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT104510.1529577888605867No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT92080.13476560327512027No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA89400.13084323341437612No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT88520.12955529107204222No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT86740.1269501349705032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)