FastQCFastQC Report
Wed 9 Apr 2025
tfl1.20.dry.SD.3.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametfl1.20.dry.SD.3.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10888386
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG496350.45585268560464337No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT282110.2590925780919229No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG265180.2435439008132151No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT199130.1828829360017178No Hit
TCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATC153760.14121468507821086No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC151860.13946970652950766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)