FastQCFastQC Report
Wed 9 Apr 2025
mA.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemA.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7560414
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT360800.4772225436331926No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210480.27839745283790013No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT158070.20907585219539565No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT136240.18020177201936294No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG128890.1704800821753941No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA103670.13712212056112272No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC102510.1355878130483331No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT97910.12950349015278792No Hit
AAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCTGC92230.12199067405568001No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT88980.11769196766208835No Hit
CACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAG88690.11730839078389094No Hit
CACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCTGCGCAACTCT83720.11073467669892151No Hit
CAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGAC81600.1079305974514094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)