FastQCFastQC Report
Wed 9 Apr 2025
mA.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemA.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11388318
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT389330.34186786845959166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269740.23685675092669523No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT269300.23647039009623722No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC258580.22705723531780547No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG208570.1831438145650657No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT202860.1781299046970764No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA200420.1759873582736274No Hit
CACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAG196310.17237839688003093No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT185270.16268425240672063No Hit
CAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGAC155550.13658733449487448No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT138360.1214929193231169No Hit
CACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCTGCGCAACTCT130760.11481941406975113No Hit
TCAAAGTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAA127120.11162315629050752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)