FastQCFastQC Report
Wed 9 Apr 2025
mA.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemA.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11119704
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT272750.2452853061556315No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG253500.22797369426380415No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT245550.2208242233786079No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC243330.21882776735783613No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT195910.1761827473105399No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181960.16363744934217672No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT140990.12679294340928499No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA122250.10993997681952684No Hit
TCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATC118790.10682838320156725No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT116340.1046250871426074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)