FastQCFastQC Report
Wed 9 Apr 2025
mB.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemB.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6691059
Total Bases979.7 Mbp
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACTTTATAATTAAGCATTAAGAGCTCTTGGTAGATTAACATGGGAAG82220.12288039905192884No Hit
GTCCCACTTGGCCTCTCGCACAACTTCTGTGCTTCCACCACCATCGGTGC77400.11567675610094008No Hit
CTCATTATTTTTATTTAATATGTATACACGATTGTTCGAAGCTTAACGGC73830.11034127781566416No Hit
CCTCATTATTTTTATTTAATATGTATACACGATTGTTCGAAGCTTAACGG67330.1006268215539573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)