FastQCFastQC Report
Wed 9 Apr 2025
mB.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamemB.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6691059
Total Bases982.9 Mbp
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT188210.2812858173870534No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG141330.2112221697641584No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131990.19726324338195195No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT114990.1718562039282571No Hit
AGAAAACATACACAAATAGCAAAACATGGCTAACAAGCTCTTCCTCGTCT112280.16780602293299163No Hit
GTCTAATATCACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGT100570.1503050563445936No Hit
GTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAGACAAGAAGAACA93450.13966399040869315No Hit
GTTTGTTTGGTTTTTACTTGTCTAGTGTTTTATATAAACTTGTGTTGTGT90400.1351056686243538No Hit
CACAAAGAAAGAGTTTTTTTAACTAGCTTAGCTCAAAGTGTTTGCTTAAG71210.10642560467633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)