FastQCFastQC Report
Wed 9 Apr 2025
tfl1.20.dry.SD.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametfl1.20.dry.SD.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8741830
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171140.19577136594969247No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT167740.19188202012622071No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG152670.1746430667263033No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC133390.15258818805673413No Hit
TCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATC112770.12900044956262016No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT101030.11557076721922067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)