FastQCFastQC Report
Wed 9 Apr 2025
tfl1.20.dry.SD.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametfl1.20.dry.SD.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10862824
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434040.3995646067726035No Hit
ATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCAT280860.2585515516038923No Hit
CGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATCG265300.24422746792178535No Hit
TATACATCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCT198790.18300029531915457No Hit
TCGAAAACAACTAAGTATAAACAATAGTCATGGCTAAGTCTGCTACCATC154460.14219138596004133No Hit
CTCGCCAAAAACCTTGAAACCTTTAAAAAATCGAAACTATGGCTAAGCTC151440.13941126174924678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)