FastQCFastQC Report
Wed 9 Apr 2025
MM.MeSA.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMM.MeSA.2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31639579
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGGCTCATCTGGGGG3615971.142862868055229TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGGCTCATCTAGGGG2094320.6619304258125559TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)