FastQCFastQC Report
Wed 9 Apr 2025
MM.NT.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMM.NT.2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38149686
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAGTTCATCTGGGGG4218581.1057967816563419TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAGTTCATCTAGGGG4117951.079419107145469TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAGTTCATCTAGTGG491530.12884247592496567TruSeq Adapter, Index 1 (97% over 37bp)
CTTCGGGCTTGTAAGCGATGAAACTGATGCACTGCACTTGACGAACATTG466240.12221332568766097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)