FastQCFastQC Report
Wed 9 Apr 2025
MM.NT.3.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMM.NT.3.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39810457
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTCGAATCTGGGGG15307813.8451731413181216TruSeq Adapter, Index 20 (97% over 39bp)
CTTCGGGCTTGTAAGCGATGAAACTGATGCACTGCACTTGACGAACATTG558330.14024707126572297No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTCGAATCTAGGGG464770.11674570829468248TruSeq Adapter, Index 20 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)